Comparison of SIC with LC
4.1.7.
Since sample is metered by reversal movement of the syringe pump, the injected sample volume can conveniently be optimized. This feature was confirmed in a work where also comparison of SIC with HPLC was carried out (Šatínský 2003).
A Chromolith® Flash RP-18e, 25mm × 4.6mm column (Merck, Germany) was used for separations with a mobile phase of acetonitrile:water (40:70 (v/v)) + 0.05% triethylamine, pH 2.5. A gradient flow rate was applied for separation of methylparaben (MP), propylparaben (PB), and sodium dichlofenac (SD) with a butylparaben (BP) internal standard Comparison of the resulting chromatograms demonstrate that SIC yields separations that are similar to the performance of HPLC.
This finding is likely to apply to isocratic separation of a small number (~5) of components. For a larger number of components, the physical and mechanical limitations of SIC hardware (finite syringe stroke volume, low back pressure tolerance, limited gradient capability) are restricting separation efficiency.
Further examples of using SIC for pharmaceutical analysis can be found in the following papers (Chocholouš 2006, Chocholouš 2007).
Šatínský D., Solich P., Chocholouš P., Karlícek R.: Anal. Chim. Acta, 499 (2003) 205-214.
Chocholouš P., Šatínský D., Solich P.: Talanta 70 (2006) 408–413.
Chocholouš P., Holík P., Šatínský D., Solich P.: Talanta 72 (2007) 854–858.
